全文获取类型
收费全文 | 4485篇 |
免费 | 363篇 |
国内免费 | 128篇 |
出版年
2023年 | 41篇 |
2022年 | 40篇 |
2021年 | 110篇 |
2020年 | 86篇 |
2019年 | 109篇 |
2018年 | 183篇 |
2017年 | 131篇 |
2016年 | 186篇 |
2015年 | 272篇 |
2014年 | 274篇 |
2013年 | 363篇 |
2012年 | 396篇 |
2011年 | 350篇 |
2010年 | 202篇 |
2009年 | 167篇 |
2008年 | 212篇 |
2007年 | 217篇 |
2006年 | 208篇 |
2005年 | 155篇 |
2004年 | 137篇 |
2003年 | 142篇 |
2002年 | 104篇 |
2001年 | 111篇 |
2000年 | 94篇 |
1999年 | 73篇 |
1998年 | 37篇 |
1997年 | 42篇 |
1996年 | 23篇 |
1995年 | 30篇 |
1994年 | 34篇 |
1993年 | 21篇 |
1992年 | 50篇 |
1991年 | 37篇 |
1990年 | 51篇 |
1989年 | 41篇 |
1988年 | 21篇 |
1987年 | 22篇 |
1986年 | 22篇 |
1985年 | 24篇 |
1984年 | 14篇 |
1983年 | 17篇 |
1982年 | 9篇 |
1981年 | 8篇 |
1976年 | 7篇 |
1975年 | 8篇 |
1974年 | 10篇 |
1973年 | 11篇 |
1971年 | 8篇 |
1970年 | 9篇 |
1968年 | 7篇 |
排序方式: 共有4976条查询结果,搜索用时 15 毫秒
101.
Antonio Mu?oz-Mérida Juan José González-Plaza Andrés Ca?ada Ana María Blanco Maria del Carmen García-López José Manuel Rodríguez Laia Pedrola M. Dolores Sicardo M. Luisa Hernández Raúl De la Rosa Angjelina Belaj Mayte Gil-Borja Francisco Luque José Manuel Martínez-Rivas David G. Pisano Oswaldo Trelles Victoriano Valpuesta Carmen R. Beuzón 《DNA research》2013,20(1):93-108
Olive breeding programmes are focused on selecting for traits as short juvenile period, plant architecture suited for mechanical harvest, or oil characteristics, including fatty acid composition, phenolic, and volatile compounds to suit new markets. Understanding the molecular basis of these characteristics and improving the efficiency of such breeding programmes require the development of genomic information and tools. However, despite its economic relevance, genomic information on olive or closely related species is still scarce. We have applied Sanger and 454 pyrosequencing technologies to generate close to 2 million reads from 12 cDNA libraries obtained from the Picual, Arbequina, and Lechin de Sevilla cultivars and seedlings from a segregating progeny of a Picual × Arbequina cross. The libraries include fruit mesocarp and seeds at three relevant developmental stages, young stems and leaves, active juvenile and adult buds as well as dormant buds, and juvenile and adult roots. The reads were assembled by library or tissue and then assembled together into 81 020 unigenes with an average size of 496 bases. Here, we report their assembly and their functional annotation. 相似文献
102.
103.
104.
Santini Nadia S. Lovelock Catherine E. Hua Quan Zawadzki Atun Mazumder Debashish Mercer Tim R. Muñoz-Rojas Miriam Hardwick Simon A. Madala Bindu Swapna Cornwell William Thomas Torsten Marzinelli Ezequiel M. Adam Paul Paul Swapan Vergés Adriana 《Ecosystems》2019,22(8):1803-1822
Ecosystems - Saltmarshes provide many valuable ecosystem services including storage of a large amount of ‘blue carbon’ within their soils. To date, up to 50% of the world’s... 相似文献
105.
106.
107.
Haiman Mu Haiwen Liu Jiayi Zhang Jianhua Huang Chen Zhu Yue Lu Yueping Shi Yi Wang 《Journal of cellular and molecular medicine》2019,23(3):2174-2183
In addition to the known antitumour effects of ursolic acid (UA), increasing evidence indicates that this molecule plays a role in cardiac protection. In this study, the effects of ursolic acid on the heart in mice treated with doxorubicin (DOX) were assessed. The results showed that ursolic acid improved left ventrical fractional shortening (LVFS) and left ventrical ejection fraction (LVEF) of the heart, increased nitrogen oxide (NO) levels, inhibited reactive oxygen species (ROS) production and decreased cardiac apoptosis in mice treated with doxorubicin. Mechanistically, ursolic acid increased AKT and endothelial nitric‐oxide synthase (eNOS) phosphorylation levels, and enhanced eNOS expression, while inhibiting doxorubicin induced eNOS uncoupling through NADPH oxidase 4 (NOX4) down‐regulation. These effects of ursolic acid resulted in heart protection from doxorubicin‐induced injury. Therefore, ursolic acid may be considered a potential therapeutic agent for doxorubicin‐associated cardiac toxicity in clinical practice. 相似文献
108.
Ji-Gang Zhang Dan-Dan Zhang Ying Liu Juan-Ni Hu Xue Zhang Li Li Wan Mu Guan-Hua Zhu Qin Li Gao-Lin Liu 《生物化学与生物物理学报:疾病的分子基础》2019,1865(6):1113-1125
Vasculogenic mimicry (VM) results in the formation of an alternative circulatory system that can improve the blood supply to multiple malignant tumors, including hepatocellular carcinoma (HCC). However, the potential mechanisms of RhoC/ROCK in VM have not yet been investigated in HCC. Here, RhoC expression was upregulated in HCC tissues, especially the VM-positive (VM+) group, compared to noncancerous tissues (P < 0.01), and patients with high expression of RhoC had shorter survival times (P < 0.001). The knockdown of RhoC via short hairpin RNA (shRNA) in SK-Hep-1 cells significantly decreased VM formation and cell motility. In contrast, cell motility and VM formation were remarkably enhanced when RhoC was overexpressed in HepG2 cells. To further assess the potential role of ROCK1 and ROCK2 on VM, we stably knocked down ROCK1 or ROCK2 in MHCC97H cells. Compared to ROCK1 shRNA, ROCK2 shRNA could largely affect VM formation, cell motility and the key VM factors, as well as the epithelial-mesenchymal transition (EMT) markers in vitro and in vivo. Moreover, p-ERK, p-MEK, p-FAK, p-paxillin, MT1-MMP and MMP2 levels were clearly altered following the overexpression of RhoC, but ROCK2 shRNA had little effect on the expression of p-FAK, which indicated that RhoC regulates FAK/paxillin signaling, but not through ROCK2. In conclusion, our results show that RhoC/ROCK2 may have a major effect on VM in HCC via ERK/MMPs signaling and might be a potential therapeutic target for the treatment of HCC. 相似文献
109.
Luengo Javier G. Muñoz María-Dolores Álvarez-Merz Iris Herranz Antonio S. González José C. Martín del Río Rafael Hernández-Guijo Jesús M. Solís José M. 《Amino acids》2019,51(9):1337-1351
Amino Acids - The application of high concentrations of taurine induces long-lasting potentiation of synaptic responses and axon excitability. This phenomenon seems to require the contribution of a... 相似文献
110.
Wang Xiao-Peng Ye Pin Lv Jiao Zhou Lei Qian Zhong-Yi Huang Yong-Jie Mu Zhi-Hao Wang Xie Liu Xin-jie Wan Qi Yang Zhi-Hong Wang Fang Zou Ying-Ying 《Neurochemical research》2019,44(4):978-993
Neurochemical Research - Cognitive impairment in diabetes (CID) is a severe chronic complication of diabetes mellitus (DM). It has been hypothesized that diabetes can lead to cognitive dysfunction... 相似文献